Which enzyme is not involved in DNA replication?

During DNA replication, several key enzymes play specific roles to ensure the accurate and efficient copying of the DNA molecule. Helicase is responsible for unwinding the double helix, single-strand binding proteins stabilize the unwound strands, and DNA polymerase synthesizes new DNA strands by adding nucleotides complementary to the template strand. After the new strands are synthesized, ligase comes into play by joining Okazaki fragments on the lagging strand, ensuring the continuity of the DNA molecule.

Phosphatase, on the other hand, does not participate in the replication process. This enzyme typically functions to remove phosphate groups from molecules, which is not a role required during DNA replication. Thus, it is correct to affirm that phosphatase does not take part in the complex sequence of events that occur when a cell replicates its DNA.