After extracting DNA from a human femur, which analysis step should be prioritized?

The first step that should be prioritized after extracting DNA from a human femur is to use polymerase chain reaction (PCR) to amplify the DNA. After extraction, the quantity of DNA may not be sufficient for further analysis, especially if the original sample is degraded or limited in quantity. PCR is a powerful technique that allows for the selective amplification of specific DNA sequences. By creating millions of copies of the target DNA, researchers can ensure there is enough material to conduct subsequent analyses.

Amplifying the DNA using PCR also enables scientists to obtain a clearer understanding of the genetic material and increases the chances of successful detection of specific sequences or fragments during later steps, such as gel electrophoresis or using nucleic acid probes for identification. This amplification step is critical for any downstream applications, including sequencing, cloning, or genotyping, as it sets the stage for more detailed analysis and experimentation.